Genetic detection methods employ specifically targeted assays that are extremely accurate and sensitive. Real-time Scorpions™ assays are 1,000 times more sensitive detecting the presence of organisms than standard microscopic examinations, and. take a fraction of the time that conventional plating does. Population estimates are reported within two working days.
Genetic analysis is capable of detecting the total population of a target organism, including the "viable but nonculturable" (VNC) cells, which are not detected by standard plating. Despite their inability to grow on plating media, these VNC cells remain active in wine for long periods of time and may continue to change a product through their metabolism.
ETS Laboratories’ Real-time Scorpions™ assays are based on amplification of genetic markers specific to each organism. This new, real-time analysis detects and quantifies microbial populations quickly – giving producers the ability to detect potential spoilage organisms before they cause sensory defects. An effective Brettanomyces program integrates this technology with standard culturing and 4-EP/4-EG analysis. Standard Applications
- Identify and quantify specific spoilage microorganisms
- Screen blends prior to aging or bottling
- Screen barrels as part of a standard quality assurance program
- Determine individual wine filtration requirements
- Monitor effectiveness of filtration